| 吴 婷，李东泽，刘嘉懿，陈秀琼，颜慧琼*.海藻酸盐/聚（N-异丙基丙烯酰胺）-聚赖氨酸复合
水凝胶的制备和性能[J].海南师范大学学报自科版,2025,38(2):183-191 |
| 海藻酸盐/聚（N-异丙基丙烯酰胺）-聚赖氨酸复合
水凝胶的制备和性能 |
| Preparation and Properties of Alginate/poly (N-isopropylacrylamide)-Polylysine Composite Hydrogel |
| |
| DOI：10.12051/j.issn.1674-4942.2025.02.009 |
| 中文关键词: 海藻酸盐 N-异丙基丙烯酰胺 组织工程支架 水凝胶 |
| 英文关键词: alginate N-isopropylacrylamide tissue engineering scaffold hydrogel |
| 基金项目:国家自然科学基金项目（51963009）；海南省重点研发项目（ZDYF2023SHFZ124）；海南省自然科学基金
（220MS035） |
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| 中文摘要: |
| 众所周知，单一海藻酸盐（Alg）水凝胶存在机械强度较弱，缺乏细胞识别位点，过度的
溶胀和不受控制的降解，因此无法满足理想支架的需求。为提高海藻酸盐水凝胶的性能，采用互
穿网络技术、聚赖氨酸（PLL）静电组装和羟基磷灰石/D-（+）-葡萄糖酸-δ-内酯内源交联相结合的
方法，成功构建了均质的海藻酸盐/聚N-异丙基丙烯酰胺-聚赖氨酸（Alg/PNIPAAm-PLL）复合水凝
胶。同时，研究了PNIPAAm与Alg的质量比对构建Alg/PNIPAAm-PLL复合水凝胶的微观形貌、抗
压强度、溶胀性和生物相容性的影响。结果表明，相较于单一Alg凝胶支架，PNIPAAm质量浓度为
1.5%时显著增强了Alg/PNIPAMM-PLL复合水凝胶的机械性能。此外，IPN结构的形成一定程度上
限制了Alg/PNIPAAm-PLL的过度溶胀，而且复合水凝胶各组分间的相互作用以及其自身温敏性在
溶胀性方面发挥了一定的作用。体外生物学评价实验结果表明，MC3T3-E1 细胞能够在 Alg/
PNIPAAm-PLL复合水凝胶上黏附、增殖和分化，具有良好的生物相容性，但过量的PNIPAAm会减
少细胞的生存空间，阻碍细胞生长，不利于细胞营养物质和代谢废物的转移，从而降低细胞活力。
因此，将互穿网络技术与PLL静电组装结合构筑的海藻酸水凝胶在生物医学领域具有广阔的应用
前景。 |
| 英文摘要: |
| It is well known that single alginate (Alg) hydrogels suffer from weak mechanical strength, lack of cellular recog⁃
nition sites, excessive swelling and uncontrolled degradation, and thus fail to meet the needs of ideal scaffolds. To improve the performance of alginate hydrogels, homogeneous alginate/poly-N-isopropylacrylamide-polylysine (Alg/PNIPAAmPLL) composite hydrogels were successfully constructed using a combination of interpenetrating network technology, poly
(lysine) (PLL) electrostatic assembly, and hydroxyapatite/D-(+)-glucono-delta-lactone endogenous cross-linking. Mean⁃
while, the effects of the mass ratio of PNIPAAm to Alg on the microscopic morphology, compressive strength, swelling and
biocompatibility of the constructed Alg/PNIPAAm-PLL composite hydrogels were investigated. The results showed that the
PNIPAAm mass concentration of 1.5% significantly enhanced the mechanical properties of Alg/PNIPAMM-PLL composite
hydrogels compared to single Alg gel scaffolds. In addition, the formation of IPN structure limited the excessive swelling of
Alg/PNIPAAm-PLL to a certain extent, and the interactions between the components of the composite hydrogel as well as
its own temperature sensitivity played a role in the swelling property. The results of the in vitro biological evaluation experi⁃
ments showed that MC3T3-E1 cells were able to adhere, proliferate and differentiate on the Alg/PNIPAAm-PLL composite
hydrogel with good biocompatibility, but the excessive amount of PNIPAAm would reduce the cell viability, impede the cell
growth, and be detrimental to the transfer of nutrients and metabolic wastes from the cells, thus reducing the cell viability.
Therefore, alginate hydrogels constructed by combining the interpenetrating network technology with PLL electrostatic as⁃
sembly have a broad application prospect in biomedical field. |
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