| 韩 筱1
，艾益静1
，姚昱岑1
，Mansoor Ahmad1
，王宝丽2*
，孙 伟1*.基于浒苔生物质碳的便携式电化学传感器的制备
及其对多巴胺检测的应用[J].海南师范大学学报自科版,2025,38(2):144-151 |
| 基于浒苔生物质碳的便携式电化学传感器的制备
及其对多巴胺检测的应用 |
| Portable Electrochemical Sensor Based on Enteromorpha proliferaDerived Biomass Carbon and Its Application in Dopamine Detection |
| |
| DOI：10.12051/j.issn.1674-4942.2025.02.004 |
| 中文关键词: 浒苔 生物质碳 多巴胺 电化学传感器 丝网印刷电极 |
| 英文关键词: Enteromorpha prolifera biomass carbon dopamine electrochemical sensor screen-printed carbon electrode |
| 基金项目:海南省激光技术与光电功能材料重点实验室开放课题（2024LTOM01） |
|
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| 中文摘要: |
| 多巴胺（DA）是一种广泛存在于中枢神经系统的重要神经递质，影响动物的各种动机与行
为，低水平的多巴胺与神经系统疾病的发病机制有关。因此，准确测量DA含量具有十分重要的意义。
本文以引起绿潮的海洋生物质浒苔为原料，通过一步碳化法制备了浒苔生物质碳（EPC），采用扫描电子
显微镜、透射电子显微镜及N2-吸附脱附等方法对其形貌及结构进行表征。结果发现所制备材料具有
介孔结构和较大的比表面积，为DA分子的吸附提供了更大的活性位点，有利于DA分子在EPC表面的
扩散。将EPC修饰到丝网印刷碳电极（SPCE）表面制成修饰电极（EPC/SPCE），利用蓝牙将智能手机与
掌上电化学工作站连接，制备了一种便携式电化学传感器并用于检测DA。结果表明，800 ℃下碳化的
800-EPC具有最强的电化学响应，在最优的检测条件下，所制备的传感器可以对10 μmol/L ~ 10 mmol/L
浓度范围内的DA进行快速灵敏检测，检出限为3.3 μmol/L（3δ）。在多种干扰物质如抗坏血酸、尿酸、氯
离子、铜离子、钠离子存在的条件下，EPC/SPCE对DA仍具有高的选择性。并且该传感器具有较好的稳
定性，可对盐酸多巴胺注射液中的DA进行定量分析，具有现场检测药物中DA含量的潜力。 |
| 英文摘要: |
| Dopamine (DA) is an important neurotransmitter widely present in the central nervous system, affecting various
motivations and behaviors of animals. Low levels of dopamine are associated with the pathogenesis of nervous system diseases.
Thus, it is very important to measure DA accurately. In this paper, Enteromorpha prolifera biomass carbon (EPC) was prepared by
one-step carbonization method from marine biomass enteromorpha which caused green tide. It was characterized by SEM, TEM and N2-adsorption and desorption. The results show that the prepared material has a mesoporous structure and a large specific
surface area, which provides a larger active sites for the adsorption of DA molecules, and is conducive to the diffusion of DA
molecules on the EPC surface. It was modified onto the surface of the screen-printed carbon electrode (SPCE) to make a
modified electrode (EPC/SPCE). A portable electrochemical sensor was prepared and used to detect DA by connecting a smart
phone to a handheld electrochemical workstation using Bluetooth. The results show that the 800-EPC carbonized at 800 ℃ has
the strongest electrochemical response. Under the optimal detection conditions, the prepared sensor can quickly and sensitively
detect DA in the concentration range of 10 μmol/L to 10 mmol/L, and the detection limit is 3.3 μmol/L (3δ). In the presence of
various interfering substances such as ascorbic acid, uric acid, chloride ion, copper ion, sodium ion, EPC/SPCE still has high
selectivity for DA. And the sensor has good stability, can be used for quantitative analysis of DA in dopamine hydrochloride
injection, and has the potential of field detection of DA content in drugs. |
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