2株内生细菌介导的木麻黄代谢组学分析及其化感潜力测定

王影; 陈盼; 林奇; 左林芝; 李蕾

文章摘要

王影, 陈盼, 林奇, 左林芝, 李蕾.2株内生细菌介导的木麻黄代谢组学分析及其化感潜力测定[J].海南师范大学学报自科版,2024,37(4):464-474

2株内生细菌介导的木麻黄代谢组学分析及其化感潜力测定

Metabolomics Analysis and Allelopathic Potential Determination of Casuarina equisetifolia Infected by Two Strains of Endophytic Bacteria

DOI：10.12051/j.issn.1674-4942.2024.04.009

中文关键词: 木麻黄解淀粉芽孢杆菌阿氏芽孢杆菌代谢组学化感作用

英文关键词: metabolomics allelopathy

基金项目:海南省院士创新平台项目（YSPTZX202129）；海南省自然科学基金项目（320QN254）

作者	单位
王影, 陈盼, 林奇, 左林芝, 李蕾	海南师范大学生命科学学院，热带岛屿生态学教育部重点实验室，海南省热带动植物生态学重点实验室，海南海口 571158

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中文摘要:

为探究木麻黄内生菌是否参与调控宿主的代谢和化感作用，采用木麻黄根内生菌阿氏芽孢杆菌XAG3和解淀粉芽孢杆菌XYG6侵染木麻黄幼苗，通过非靶向代谢组学分析侵染后幼苗与无菌苗的代谢物差异，并对差异代谢物进行化感潜力测定。代谢组学分析结果显示，XAG3和XYG6侵染后，木麻黄幼苗中柚皮素、儿茶素没食子酸酯和山柰酚-3-葡萄糖醛酸苷等代谢物的表达量均下调。化感潜力测定结果显示，柚皮素、儿茶素没食子酸酯和山柰酚-3-葡萄糖醛酸苷均能抑制木麻黄种子萌发。内生细菌XAG3和XYG6可通过异黄酮生物合成通路、类黄酮生物合成通路和酚类物质代谢通路等途径调控木麻黄次生代谢物的种类和含量，进而缓解宿主的化感作用，有助于木麻黄的自我更新。

英文摘要:

To investigate whether endophytic bacteria in Casuarina equisetifolia participate in the regulation of host metabolism and allelopathy, the study used isolated endophytic bacteria Bacillus aryabhattai XAG3 and Bacillus amyloliquefaciens XYG6 from the roots of Casuarina equisetifolia to infect Casuarina equisetifolia seedlings. Non-targeted metabolomics was used to analyze the differences in metabolites/pathways between infected and sterile seedlings, and the allelopathic potential of the differential metabolites was determined. Metabolome analysis showed that Naringenin, (-)-Catechin gallate and Kaempferol-3-Glucuronide were down-regulated in Casuarina equisetifolia seedlings after infection with XAG3 and XYG6. The determination of allelopathic potential showed that Naringenin, (-)-Catechin gallate and Kaempferol-3-Glucuronide could inhibit the germination of Casuarina equisetifolia seeds. Endophytic bacteria XAG3 and XYG6 can regulate the types and contents of secondary metabolites in Casuarina equisetifolia through pathways such as isoflavone biosynthesis, flavonoid biosynthesis, and phenolic metabolism, thereby alleviating host allelopathy and promoting the self-renewal of Casuarina equisetifolia.

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