| 任平国, 樊军浩, 徐启红.超声提取大豆皂苷工艺优化及其抗氧化活性[J].海南师范大学学报自科版,2024,37(4):444-450 |
| 超声提取大豆皂苷工艺优化及其抗氧化活性 |
| Study on Optimization of Ultrasonic Extraction Process and Antioxidant Activity of Soybean Saponins |
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| DOI：10.12051/j.issn.1674-4942.2024.04.006 |
| 中文关键词: 大豆皂苷 超声提取 响应面 工艺优化 抗氧化活性 |
| 英文关键词: soybean saponins ultrasonic extraction response surface process optimization antioxidant activity |
| 基金项目:2023年度漯河市重大科技创新专项项目计划（漯科〔2023〕51号） |
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| 摘要点击次数: 725 |
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| 中文摘要: |
| 本研究以市售国产大豆为原料，通过对超声提取大豆皂苷的影响因素进行优化，获取大豆皂苷最佳超声提取条件，分析所得大豆皂苷的抗氧化活性。在单因素影响试验分析基础上，以大豆皂苷得率为考察指标，对影响超声提取大豆皂苷的液料比、超声提取时间、乙醇体积百分比等因素进行响应面优化分析，得到超声提取大豆皂苷最佳工艺条件，并对大豆皂苷清除DPPH自由基和ABTS自由基的能力进行讨论。研究结果表明，大豆皂苷最佳超声提取条件如下：液料比为45∶1（mL∶g），超声提取时间为40 min，乙醇体积百分比为66%，在此最佳超声提取条件下，进行3次平行试验，所得大豆皂苷得率平均值为（4.21±0.13） mg/g，与响应面优化分析的预测值4.22 mg/g基本吻合，所得大豆皂苷对DPPH自由基和ABTS自由基的IC50值分别为（0.44±0.06）mg/mL和（0.39±0.07）mg/mL。本研究结果表明优化后的超声提取大豆皂苷工艺参数可靠，提取得到的大豆皂苷具有较为理想的抗氧化活性，可在食品生产和饲料加工中作为天然抗氧化剂应用。本研究结果对大豆皂苷的工业化提取和大豆资源的深度开发及利用提供理论依据。 |
| 英文摘要: |
| In this study, the commercial domestic soybeans were used as raw materials to study the optimal ultrasonic extraction conditions of soybean saponin and its antioxidant activity. Based on the analysis of single factor effect test, the extraction rate of soybean saponin was used as the index to optimize the response surface analysis of the factors affecting the liquid-solid ratio, ultrasonic extraction time and ethanol volume percentage of soybean saponin extraction. The optimal process conditions for ultrasonic extraction of soybean saponin were obtained, and the ability of soybean saponin to remove DPPH free radicals and ABTS free radicals was discussed. The results showed that the optimum ultrasonic extraction conditions of soybean saponins were as follows: liquid-solid ratio was 45∶1 (mL∶g), ultrasonic extraction time was 40 min, and ethanol volume percentage was 66%. Under the optimal ultrasonic extraction conditions, the average yield of soybean saponins obtained by three parallel experiments was (4.21 ± 0.13) mg/g, which was basically consistent with the predicted value of 4.22 mg/g by response surface optimization analysis. The IC50 values of soybean saponins against DPPH and ABTS free radicals were (0.44 ± 0.06) mg/mL and (0.39 ± 0.07) mg/mL, respectively. The results showed that the optimized ultrasonic extraction process parameters of soybean saponins were reliable. The extracted soybean saponins had ideal antioxidant activity, which could be used as a natural antioxidant in food production and feed processing. The research results provide a theoretical basis for the industrial extraction of soybean saponins and the deep development and utilization of soybean resources. |
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