HPLC法同时测定植物饮料中7种绿原酸

黄柳霞<sup>1; 2; 3</sup>; 许雪儿<sup>1; 2; 3</sup>; 李莲<sup>1; 2; 3</sup>

文章摘要

黄柳霞, 许雪儿, 李莲.HPLC法同时测定植物饮料中7种绿原酸[J].海南师范大学学报自科版,2024,37(2):218-223

HPLC法同时测定植物饮料中7种绿原酸

Simultaneous Determination of Seven Chlorogenic Acids in Plant Vegetable Beverages by HPLC

DOI：10.12051/j.issn.1674-4942.2024.02.012

中文关键词: 高效液相色谱法植物饮料 7种绿原酸

英文关键词: HPLC vegetable beverages seven chlorogenic acids

基金项目:广东省食品质量安全服务工程技术研究中心（粤科函产字［2020］141号）

作者	单位
黄柳霞^1,2,3, 许雪儿^1,2,3, 李莲^1,2,3	1.广东省食品工业公共实验室，广东　广州　 511446 2.广东省食品工业研究所有限公司，广东　广州　 511446 3.广东省质量监督食品检验站，广东　广州　 511446

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中文摘要:

建立一种同时测定植物饮料中7种绿原酸的HPLC法：采用C₁₈色谱柱，以乙腈+0.1%甲酸+0.1%乙酸作为流动相进行梯度洗脱，检测波长为327 nm，参比波长400 nm。7种绿原酸检出限为0.25 mg/L，在0.5~20 μg/mL浓度范围内成线性关系，新绿原酸、绿原酸、隐绿原酸、异绿原酸B、1，5-二咖啡酰奎尼酸、异绿原酸A、异绿原酸C回收率分别为99.0%~108%、97.9%~101%、96.9%~102%、96.2%~106%、95.1%~96.7%、94.9%~99.6%、96.3%~108%。该方法简单、快捷、定性定量准确，可同时测定植物饮料中7种绿原酸。

英文摘要:

An HPLC method was established for the simultaneous determination of seven chlorogenic acids in plant beverages, by using C₁₈ chromatographic column, elutioned with acetonitrile + 0.1 % formic acid + 0.1 % acetic acid . The detection wavelength was 327 nm and the reference wavelength was 400 nm. The detection limit of seven chlorogenic acids was 0.25 mg/L, and the relationship was linear in the concentration range of 0.5~20 μg/mL. The recoveries of neochloro genic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, 1, 5-dicaveoyl quinic acid, isochlorogenic acid A and isochlorogenic acid C were 99%~108%, 97.9%~101%, 96.9%~102%, 96.2%~106%, 95.1%~96.7%, 94.9%~99.6%, 96.3%~108%, respectively. The method was simple, fast, accurate in qualitative and quantitative, and could simultaneously determine seven kinds of chlorogenic acids in plant beverages.

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