| 王志宇, 沈舒婷, 杨静, 胡磊, 王慧.基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测[J].海南师范大学学报自科版,2024,37(2):158-163 |
| 基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测 |
| A Fluorescent Probe Based on Isophorone Dicyanide for the Detection of Cysteine in Cells |
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| DOI：10.12051/j.issn.1674-4942.2024.02.004 |
| 中文关键词: 半胱氨酸 荧光探针 丙烯酰氯 二氰基异佛尔酮 |
| 英文关键词: cysteine fluorescent probe acryloyl chloride isophorone dicyano |
| 基金项目:安徽省自然科学基金项目（1908085QB50）；安徽省大学生创新创业教育训练计划项目（S202310368089） |
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| 中文摘要: |
| 半胱氨酸（Cys）在生物体系中起着不可缺少的作用，可以参与人体内的许多生理和病理过程，对氧化还原稳态和细胞活性的调节有重要作用。大量研究表明，Cys浓度异常与许多疾病的发生密不可分，因此有必要开发特异性检测Cys的荧光探针。本文以丙烯酰基为识别单元，以二氰基异佛尔酮为荧光基团，设计合成了一种荧光探针ISO-EV-AC。实验结果表明，探针ISO-EV-AC对Cys的检测展现出较高的选择性，不受其他分析物的干扰，检测限低至31 nmol/L，响应Cys的时间为5 min。细胞实验结果表明探针ISO-EV-AC可用于检测细胞中的Cys。 |
| 英文摘要: |
| Cysteine (Cys) plays an indispensable role in biological systems, which can participate in a variety of physiological and pathological processes in the human body and plays an essential role in the regulation of redox homeostasis and cell activity. A large number of studies have shown that abnormal Cys concentrations are strongly associated with the occurrence of various diseases. Therefore, it is necessary to develop a specific fluorescence probe for Cys detection. In this paper, a fluorescent probe ISO-EV-AC was designed and synthesized using acryloyl group as the recognition unit and isophorone dicyano as the fluorophore. The results indicated that probe ISO-EV-AC exhibits a high selectivity for the detection of Cys without interference from the other nanlytes. The detection limit was as low as 31 nmol/L and the response time of Cys was 5 min. The results of the cell experiments demonstrated that probe ISO-EV-AC can be used to detect Cys in cells. |
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