| 陈炜康1,2
，罗运展1,2
，陈世季1,2
，黄国雷1,2
，李诗川3
，郑彩娟1,2.一株海泥来源真菌Talaromyces flavus 210331次级
代谢产物及其抗氧化活性[J].海南师范大学学报自科版,2023,36(2):138-142 |
| 一株海泥来源真菌Talaromyces flavus 210331次级
代谢产物及其抗氧化活性 |
| Secondary Metabolites and Their Antioxidant Activities of a Sea Mud-derived Fungus Talaromyces flavus 210331 |
| |
| DOI：10.12051/j.issn.1674-4942.2023.02.005 |
| 中文关键词: 海泥 Talaromyces flavus 次级代谢产物 抗氧化活性 |
| 英文关键词: sea mud Talaromyces flavus secondary metabolites antioxidant activity |
| 基金项目:海南省重大科技计划项目（ZDKJ202008）；国家自然科学基金项目（32160108）；海南省院士创新平台科研专项
（YSPTZX202030） |
|
| 摘要点击次数: 623 |
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| 中文摘要: |
| 为从Talaromyces 属中获得更多生物活性显著的次级代谢产物，对一株红树植物白骨
壤（Avicennia marina）根际海泥来源真菌Talaromyces flavus 210331的次级代谢产物及其抗氧化活性
进行研究。综合利用正相硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备HPLC等色谱分离技
术，对该菌发酵产物的乙酸乙酯萃取物进行次级代谢产物分离纯化；综合运用NMR、MS波谱对纯
化的次级代谢产物进行结构鉴定，并评价鉴定次级代谢产物的抗氧化活性。结果显示从真菌T.
flavus 210331 中分离鉴定 7 个单体化合物，包括 2 个异香豆素类化合物 hypoxymarin C（1）和
peniciisocoumarin F（2），1个联烯类化合物penicilactone A（3），4个苯衍生物diorcinol（4）、p-hydroxybenzaldenhyde（5）、trans-ferulic acid（6）和agidol 10（7）。抗氧化活性测试结果表明化合物1~3具有
显著的抗氧化活性，其IC50值分别为（0.180±0.025）、（0.180±0.037）和（0.230±0.013） mmol/L，强于
阳性对照trolox［IC50=（0.290±0.011） mmol/L］。 |
| 英文摘要: |
| In order to obtain more secondary metabolites with significant biological activities from the genus Talaromyces,
the secondary metabolites and biological activities of the fungus Talaromyces flavus 210331 isolated from sea mud of the
rhizosphere of Avicennia marina were studied. The secondary metabolites were isolated and purified by normal phase silica gel column chromatography, Sephadex LH-20 gel column chromatography, semi-preparative HPLC and other chro‑
matographic separation methods from the EtOAc extracts. Their structures were identified by comprehensive NMR, MS
spectral and other spectral analysis methods. The antioxidant activities of the isolated secondary metabolites were also
tested. Seven compounds were isolated and identified from T. flavus 210331, including two isocoumarins hypoxymarin C
(1), peniciisocoumarin F (2) a diene penicilactone A (3), four benzene derivatives diorcinol (4), p-hydroxy-benzaldenhyde
(5), trans-ferulic acid (6), and agidol 10 (7). Compounds 1-3 showed good antioxidant activity, with the IC50 values of
0.18±0.025, 0.18±0.037 and 0.23±0.013mmol/L, respectively, better than positive control trolox with the IC50 value of
0.29±0.011 mmol/L。 |
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