| 李 莲1,2,3
，黄柳霞1,2,3
，罗雪婷1,2,3.二维液相色谱法同时测定婴幼儿配方奶粉中的
维生素A、D、E[J].海南师范大学学报自科版,2023,36(1):46-52 |
| 二维液相色谱法同时测定婴幼儿配方奶粉中的
维生素A、D、E |
| Simultaneous Determination of Vitamin A, D and E in Powdered Infant Formula by Two-dimensional Liquid Chromatography |
| |
| DOI：10.12051/j.issn.1674-4942.2023.01.007 |
| 中文关键词: 二维液相色谱法 维生素A 维生素D 维生素E 婴幼儿配方奶粉 |
| 英文关键词: two-dimensional liquid chromatography vitamin A vitamin D vitamin E infant formula |
| 基金项目: |
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| 摘要点击次数: 453 |
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| 中文摘要: |
| 本文建立了二维液相色谱法同时测定婴幼儿配方奶粉中维生素A、E、D含量。样品经
皂化、萃取、浓缩、定容，经二维反相色谱法测定，对所建立的方法进行方法学验证，结果表明：维生
素A、D、E标准曲线线性良好，R2
>0.999，维生素A、E、D检出限分别为8、0.65、30 μg/100 g；定量限分
别为25、2、100 μg/100 g，加标回收率为87.3%~96.5%，RSD为0.45%~3.80%，同时与国标法进行比
较，该方法更加简便快捷，灵敏度高，结果也相对较准。 |
| 英文摘要: |
| In this paper, two-dimensional liquid chromatography was established to simultaneously determine the content
of vitamins A, E and D in powdered infant formula. The samples were saponified, extracted, concentrated, volumed, and⁃
determined by two-dimensional reversed-phase chromatography, and the established method was verified methodological⁃
ly, and the results showed that the standard curves of vitamin A, D and E were linear with R2
>0.999, the detection limits of
vitamins A, E and D were 8, 0.65, 30 μg/100 g. The quantitative limits were 25, 2, 100 μg/100 g, the recovery rate of
spiked were 87.3%~96.5%, and the RSD were 0.45%~3.8%. Compared with the national standard method, the method was
simpler and faster, with high sensitivity and relatively accurate results. |
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