| 黄柳霞1，2，3
，陈 茹1，2，3
，张 琴1，2，3
，陈丽香1，2，3
，冯志强1，2，3.高效液相色谱法同时测定蜂王浆口服液中5-羟甲基
糠醛和10-羟基-2-癸烯酸方法研究[J].海南师范大学学报自科版,2022,35(4):407-411 |
| 高效液相色谱法同时测定蜂王浆口服液中5-羟甲基
糠醛和10-羟基-2-癸烯酸方法研究 |
| Study on Simultaneous Determination of 5-Hydroxymethylfurfuraland 10-Hydroxy-2-Decenoic Acid in Royal JellyOral Liquid by HPLC |
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| DOI： |
| 中文关键词: 蜂王浆口服液 5-羟甲基糠醛 10-羟基-2-癸烯酸 高效液相色谱法 |
| 英文关键词: royal jelly oral liquid 5-hydroxymethylfurfural 10-hydroxy-2-decenoic acid HPLC |
| 基金项目:广东省科技计划项目（2014A040401070） |
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| 中文摘要: |
| 建立一种用反相高效液相色谱法同时测定蜂王浆口服液中5-羟甲基糠醛和10-羟基-
2-癸烯酸的方法。样品经 0.01 mol/L 的盐酸溶液和甲醇溶解并沉淀蛋白后提取。采用 C18 柱
（250 mm×4.6 mm，5 µm）分离，以甲醇（55%）+0.01 mol/L盐酸溶液（45%）为流动相，流速为1.0 mL/min，
检测波长为280 nm（5-羟甲基糠醛）及 210 nm（10-羟基-2-癸烯酸），以出峰时间定性，以外标法定
量。结果表明，该方法的检出限及定量限均为 5 mg/kg，线性范围为 0.5~20.0 µg/mL。同时测定
5-羟甲基糠醛和 10-羟基-2-癸烯酸方法的相关系数分别为 0.999 97 和 0.999 95，回收率分别为
89.4%~103.0%和88.9%~102.0%。 |
| 英文摘要: |
| A method for simultaneous determination of 5-hydroxymethylfurfural and 10-hydroxy-2-decenoic acid in roy⁃
al jelly oral liquid by reversed-phase high performance liquid chromatography was established. The samples were dissolved
in 0.01 mol/L hydrochloric acid solution and methanol, and the protein was precipitated and extracted. C18 column
(250 mm×4.6 mm, 5 µm) was used for separation, methanol (55%) + 0.01 mol/L hydrochloric acid solution (45%) was used
as mobile phase, flow rate was 1.0 mL/min, detection wavelength was 280 nm (5-hydroxymethylfurfural) and 210 nm (10-
hydroxy-2-decenoic acid), the peak time was qualitative, and the external standard method was quantitative. The results
show that the detection limit and quantification limit of this method are both 5 mg/kg, the linear range is 0.5~20.0 µg/mL,
and the correlation coefficients were 0.999 97 for 5-hydroxymethylfurfural and 0.999 95 for 10-hydroxy-2-decenoic acid.
The recoveries were 89.4%-103% and 88.9%-102% respectively. |
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