邵 波1,党雯迪1,闫丽君1,孙云秀1,施 璠1,黄玉豪1,王晓峰2,孙 伟1*.基于芯片三电极系统检测辣根过氧化物酶的研究[J].海南师范大学学报自科版,2021,34(3):279-284 |
基于芯片三电极系统检测辣根过氧化物酶的研究 |
Detection of Horseradish Peroxidase Basedon a Chip Three-electrode System |
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DOI:10.12051/j.issn.1674-4942.2021.03.005 |
中文关键词: 芯片电极 邻联茴香胺 邻苯二胺 差分脉冲伏安法 辣根过氧化物酶 |
英文关键词: chip electrode o-dianiline o-phenylenediamine differential pulse voltammetry horseradish peroxidase |
基金项目:海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC188);海南师范大学研究生创新科
研课题(hsyx2020-30);海南师范大学大学生创新训练计划项目;北京未来芯片技术创新中心课题(KYJJ2018006) |
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中文摘要: |
本文设计一种芯片三电极系统,以金盘电极为工作电极,金片电极为辅助电极,Ag/
AgCl电极为参比电极,以邻联茴香胺(ODA)和邻苯二胺(OPD)两种常见的底物体系建立了检测辣
根过氧化物酶(HRP)的方法。HRP能够催化H2O2氧化ODA和OPD,其氧化产物在芯片金电极上分
别于-0.23 V和-0.50 V(vs.Ag/AgCl)处产生一个灵敏的还原峰,峰电流随HRP浓度增加而增大。对 于 ODA-H2O2体系,测定游离 HRP 的范围是 5.0×10-7~1.0×10-5 g/mL,检出限为 1.0×10-7 g/mL
(3σ)。对于 OPD-H2O2体系,测定游离 HRP 的线性范围是 1.0×10-8~1.0×10-4 g/mL,检出限为
1.0×10-9 g/mL(3σ)。 |
英文摘要: |
A chip three-electrode system was designed with gold disc electrode as working electrode, gold slice electrode
as auxiliary electrode and Ag/AgCl electrode as reference electrode. A method for the detection of horseradish peroxidase
(HRP) was established by using o-dianiline (ODA) and o-phenylenediamine (OPD) as substrate systems. HRP catalyzed
H2O2 to oxidize ODA and OPD, and the oxidation products produced a sensitive reduction peak at -0.23 V and -0.50 V (vs.
Ag/AgCl) respectively. The peak current increased with the increase of HRP concentration. For ODA-H2O2 system, the lin⁃
ear range of free HRP was 5.0×10-7 ~ 1.0×10-5 g/mL with the detection limit as 1.0×10-7 g/mL (3σ). For OPD-H2O2 system,
the linear range of free HRP was 1.0× 10-8 ~ 1.0× 10-4 g/mL with the detection limit as 1.0×10-9 g/mL (3σ). |
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