| 邵波，闫丽君，李晓燕，朱俊怡，杨翁琳，习亚茹，党文迪，孙伟*.基于PEDOT:PSS 和HRP 的电化学酶传感器的制备与电催化应用[J].海南师范大学学报自科版,2020,33(3):243-248 |
| 基于PEDOT:PSS 和HRP 的电化学酶传感器的制备与电催化应用 |
| Preparation and Electrocatalytic Application of PEDOT:PSS and HRP Based Electrochemical Enzyme Sensor |
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| DOI：10.12051/j.issn.1674-4942.2020.03.001 |
| 中文关键词: 聚（3，4-乙烯二氧噻吩）/聚乙烯苯磺酸 辣根过氧化物酶 循环伏安法 电化学酶传
感器 |
| 英文关键词: poly(3.4-ethylene dioxythiophene)/poly(styrenesulfonate) horseradish peroxidase cyclic voltammetry electrochemical enzyme sensor |
| 基金项目:国家自然科学基金项目（21665007）；海南省基础与应用基础研究计划（自然科学领域）高层次人才项目
（2019RC188） |
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| 中文摘要: |
| 利用聚（3，4-乙烯二氧噻吩）/聚乙烯苯磺酸（PEDOT：PSS）和辣根过氧化物酶（HRP）制
备了一种电化学酶传感器。采用离子液体N-己基吡啶六氟磷酸盐（HPPF6）修饰碳糊电极（CILE）
作为基底电极，将PEDOT：PSS 和HRP 的混合材料修饰于电极表面得到电化学酶传感器（PEDOT：
PSS-HRP/CILE）。利用红外光谱法和紫外光谱法对HRP 的活性进行了分析，采用循环伏安法（CV）
对该酶传感器的直接电化学和电催化行为进行了测试。结果表明：PEDOT：PSS-HRP/CILE 对三氯
乙酸（TCA）和亚硝酸钠（NaNO2）有明显的电催化行为，当TCA 浓度在0.0～300.0 mmol/L 的范围时
还原峰电流值与浓度的变化成线性关系，检测限为0.67 mmol/L（3σ）。使用此传感器成功地对药物
样品中TCA 含量进行了测定，加标回收率为94.0%～105.6%。 |
| 英文摘要: |
| An electrochemical enzyme sensor was prepared by using poly(3.4-ethylene dioxythiophene)/poly(styrenesulfo⁃
nate) (PEDOT:PSS) and horseradish peroxidase (HRP), modified carbon ionic liquid electrode (CILE), which was fabricated
by using ionic liquid N-hexylpyridinium hexafluorophosphate (PEDOT:PSS-HRP/CILE). FT-IR and UV-Vis absorption
spectroscopy was used to check the structure of HRP. Direct electrochemical behavior and electrocatalysis of this HRP
based sensor were measured by cyclic voltammetry (CV). The results showed that PEDOT:PSS-HRP/CILE had significant
electrocatalytic activity to the reduction of trichloroacetic acid (TCA) and NaNO2. When the TCA concentration was in the
range of 0.0 ~ 300.0 mmol/L, the reduction peak current changed linearly with TCA concentration, and the detection limit
was 0.67 mmol/L (3σ). The TCA content in the drug samples was successfully determined using this sensor with the recover⁃
ies from 94.0% to 105.6%. |
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