| 吕雅娟1，袁瑞青2，李志英2*.2-巯基苯并噻唑荧光增敏法检测过氧化氢含量及过氧化氢酶活力[J].海南师范大学学报自科版,2020,33(2):132-135 |
| 2-巯基苯并噻唑荧光增敏法检测过氧化氢含量及过氧化氢酶活力 |
| Determination of Hydrogen Peroxide and Catalase Activity by Fluorescence Enhancement of 2- Mercaptobenzothiazole |
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| DOI：10.12051/j.issn.1674-4942.2020.02.003 |
| 中文关键词: 2-巯基苯并噻唑 荧光增敏法 过氧化氢酶 |
| 英文关键词: 2-mercaptobenzothiazole fluorescence enhancement method hydrogen peroxide catalase |
| 基金项目:山西省1331工程学科建设计划经费资助（2018122） |
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| 中文摘要: |
| 2-巯基苯并噻唑溶液有微弱的荧光，加入过氧化氢溶液荧光增强。由此建立测定过氧化氢含量的新方法。结果表明：测定最佳条件为：pH = 7，温度为55 ℃下反应30 min。过氧化氢含量在0.23 ~ 116 m mol/L 范围内呈良好的线性关系，工作曲线ΔF = 6.69 c + 67.26（m mol/L），该方法的相关系数r = 0.9962，检出限为6.73×10-5 mol/L。在此基础上可以测定蔬菜中过氧化氢酶活性。该方法体系简单、方便、准确可靠，可作为蔬菜中过氧化氢酶活性的测定方法。 |
| 英文摘要: |
| A new method was established to test the content of catalase activity in vegetables. The fluorescence of 2-Mercaptobenzothiazole
solution is weak, and it is enhanced by adding hydrogen peroxide solution. A new method for the determination
of hydrogen peroxide was developed. The results showed that the optimum conditions were pH =7 and the temperature
55 ℃ for 30 min. Under optimal conditions, the concentration of H2O2 and ΔF showed a good linear relationship in the
range of 0.23～116 mmol/L, the work curve was ΔF = 6.69 c + 67.26 mmol/L. The correlation coefficient (r) was 0.9962
and the detection limit was 6.73 × 10-5 mol/L. On this basis, catalase activity in vegetables was determined. The method of
system is simple, convenient , accurate and reliable and can be used for determination of the catalase activity in vegetables. |
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