| 曾斯杰1，3，马金爽1，王 玥1，刘彦弟1，尹 星1，韩长日2，杨定国2，刘 红1*.基于近红外光谱技术快速检测青金桔果粉中微生物菌数[J].海南师范大学学报自科版,2020,33(1):30-35 |
| 基于近红外光谱技术快速检测青金桔果粉中微生物菌数 |
| Detection of Total Microorganism Counts in Kumquat Powder byNear-infrared Spectroscopy |
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| DOI：10.12051/j.issn.1674-4942.2020.01.007 |
| 中文关键词: 青金桔粉 霉菌 酵母菌 大肠杆菌 近红外光谱 |
| 英文关键词: green kumquat powder mould yeast Escherichia coli near infrared spectroscopy |
| 基金项目:海南省社会发展专项（2015SF11），海口市应用技术研究与开发项目（2016013），海南师范大学创新训练计划
项目 |
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| 摘要点击次数: 504 |
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| 中文摘要: |
| 应用近红外光谱测定及细菌培养平板计数的方法建立水分活度为0.1的青金桔原粉中
大肠杆菌、酵母、霉菌总数的快速检测模型。通过考察温度0 ℃、27 ℃、35 ℃的不同保存条件下青
金桔粉中霉菌、酵母、大肠杆菌的近红外光谱以及微生物菌数变化，建立青金桔粉中菌落数的近红
外模型。结果表明：霉菌总数的校正和预测模型的有效决定系数分别为0.9811和0.9823，酵母菌总
数的校正和预测模型有效决定系数分别为0.8794和0.8264，大肠杆菌总数的校正和预测模型的有
效决定系数分别为0.9420和0.9904，实验研究的模型对青金桔粉中大肠杆菌和霉菌总数的检测准
确性较高，可用于快速、无损检测。 |
| 英文摘要: |
| The method of near infrared spectroscopy and bacterial culture plate count method was used to determine the to⁃
tal bacterial activity of kumquat powder. First, weighed the amount of green kumquat powder, in the water activity of 0.1 un⁃
der the conditions of mixing and evenly spread in a dry culture dish, the temperature 0 ℃, 27 ℃, 35 ℃ under different pres⁃
ervation conditions for parallel determination, the establishment of the total number of moulds,yeasts and Escherichia coli in
the samples were determined by plate colony counting method, and the total number of colonies in the green kumquat was
established by the near-infrared spectroscopy of the moulds, yeast, E.coli. The correlation coefficients of calibration and prediction for mould were 0.9811 and 0.9823 respectively. The correlation coefficients of calibration and prediction for
yeast were 0.8794 and 0.8264 respectively. The correlation coefficients of calibration and prediction for E.coli were 0.9420
and 0.9904 respectively.Variable near infrared model for E.coli and mould can achieve the fast, non-destructive testing of
bacteria in the green kumquat powder. |
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