| 陈翔宇，邹怡然，蒋天宁，朱宸希，陈文伟.响应面法优化碱溶酸沉法提取黄秋葵蛋白工艺条件
研究[J].海南师范大学学报自科版,2019,32(3):288-294 |
| 响应面法优化碱溶酸沉法提取黄秋葵蛋白工艺条件
研究 |
| Process Optimization for Extracting Crude Protein from Okra byAlkali-Soluble Acid Sedimentation |
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| DOI：10.12051/j.issn.1674-4942.2019.03.007 |
| 中文关键词: 黄秋葵 蛋白 碱溶酸沉 响应面优化 |
| 英文关键词: Okra protein alkali-solution and acid-isolation method response surface optimization |
| 基金项目:国家级大学生创新创业训练计划项目（201710356007）；浙江省基础公益研究计划项目（LGN18C200025） |
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| 中文摘要: |
| 黄秋葵是最佳的绿色食品之一，具有较高的营养食用价值。本研究采用碱溶酸沉法提
取黄秋葵蛋白，在单因素实验的基础上，通过响应面法对黄秋葵蛋白的提取工艺进行优化。结果
表明，优化的提取工艺为：料液比1∶52，pH值9.0，提取时间48 min，提取温度48 ℃，此条件下的黄
秋葵蛋白提取率为8.5 mg/g，为工业上提取黄秋葵蛋白提供一定的技术基础。 |
| 英文摘要: |
| Okra is one of the best green foods with high nutritional value. In this study, the okra protein was extracted by
alkali-solution and acid-isolation method. On the basis of single factor experiment, the extraction process of okra protein
was optimized by response surface methodology. The results showed that the optimized extraction process was: ratio of mate⁃
rial to liquid 1:52, pH 9.0, extraction time 48 min, extraction temperature 48 °C, and the extraction rate of okra protein was
8.5 mg/g, providing a certain technical basis for industrial extraction of okra protein. |
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