| 刘艳萍，魏 书.茶树糖基转移酶基因CsUGT73D1的研究[J].海南师范大学学报自科版,2019,32(3):268-280 |
| 茶树糖基转移酶基因CsUGT73D1的研究 |
| Studies on Glucosyltransferase CsUGT73D1 Gene in Camellia sinensis |
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| DOI：10.12051/j.issn.1674-4942.2019.03.005 |
| 中文关键词: 茶树 糖基转移酶 CsUGT73D1 转基因 |
| 英文关键词: Camellia sinensis glycosyltransferase CsUGT73D1 transgenic |
| 基金项目:国家自然科学基金资助项目（31770734） |
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| 中文摘要: |
| 茶树鲜叶中，对茶饮色、香、味品质形成具有重要作用的风味化合物，常由糖基转移酶
催化、生成糖苷化合物。其中，一些物质的合成受茉莉酸甲酯（MeJA）的诱导。本研究基于茶树转
录组数据库，发现糖基转移酶基因CsUGT73D1为MeJA诱导。该基因编码序列长1470bp，编码具
有 489 个氨基酸残基的糖基转移酶，与葡萄、拟南芥和可可同源基因（VvUGT73D1、AtUGT73D1、 TcUGT73D1）在系统进化分类上同属一组。实时定量PCR结果表明，CsUGT73D1基因在叶、雌蕊、
花瓣和雄蕊中都有表达，但表达模式不同；其表达受MeJA、GA和ABA诱导。CsUGT73D1与GFP融
合蛋白的瞬时表达结果表明，该蛋白定位于细胞质中。过表达该基因的转基因烟草和拟南芥生长
发育进程加快、叶片腺毛减少。此外，转基因烟草叶片的分泌型腺毛数量比对照叶明显减少。因
而推测，CsUGT73D1作用于多个生物学途径。 |
| 英文摘要: |
| In fresh leaves of tea tree, the flavor compounds play an important role in the formation of color, aroma and taste
quality of tea leaves, which are often catalyzed by glycosyltransferase to generate glycoside compounds. The synthesis of
some substances is induced by methyl jasmonate (MeJA). In this study, based on the transcriptome database of tea tree, it
was found that sugar transferase gene CsUGT73D1 was induced by MeJA. The cDNA sequence of this gene is 1470 bp and
encodes a glycosyltransferase with 489 amino acid residues, belonging to the same group of grape, arabidopsis and cocoa ho⁃
mologous genes (VvUGT73D1, AtUGT73D1 and TcUGT73D1) in phylogenetic classification. The results of real-time quanti⁃
tative PCR showed that CsUGT73D1 gene expressed in leaves, pistils, petals and stamens and induced by MeJA, GA and
ABA, but the expression patterns were different. The transient expression of CsUGT73D1 and GFP fusion protein indicated
that the protein was located in the cytoplasm. The transgenic tobacco and arabidopsis plantshad faster growth and less glan⁃
dular hair in the leaves aftergene overexpression. Besides, it was found that the number of secretory glandular hairs in the
leaves of transgenic tobacco was significantly lower than that in the control leaves. Therefore, it is speculated that
CsUGT73D1 play roles in multiple biological pathways. |
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