| 王松文1,2，程萌杰1，施利利1,2，孙 宁1，张 欣1,2，
丁得亮1,2，崔 晶1,2，边嘉宾1,2.水稻产量基因网络的初步研究[J].海南师范大学学报自科版,2019,32(2):163-169 |
| 水稻产量基因网络的初步研究 |
| Preliminary Studies on Rice Yield Gene Network |
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| DOI：10.12051/j.issn.1674-4942.2019.02.010 |
| 中文关键词: 水稻 产量 蛋白质组 基因网络 |
| 英文关键词: rice yield proteome gene network |
| 基金项目:国家水稻基因组计划重大专项（2017GK10102）；天津市科技计划项目（16ZXZYNC00110） |
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| 中文摘要: |
| 产量是作物育种的首要目标。采用多个品种、多个对照品种对其蛋白质组学进行了解
析，iTRAQ分析表明，水稻叶片和幼穗中有数万个功能蛋白表达，按照表达强度和产量的关联程
度，鉴定了33个对产量有重要贡献的蛋白质，其中淀粉合成酶、种子储藏蛋白是产量构成的重要成
分，采用酶活性、产量形成对糖苷合成酶及其基因调控网络进行了验证，对重要农艺性状基因克
隆、基因网络重构等进行了讨论。 |
| 英文摘要: |
| Yield is the primary goal of crop breeding. Proteomics was analyzed using difference varieties and multiple con⁃
trol varieties. iTRAQ analysis showed that there were tens of thousands of functional proteins expressed in rice leaves and
young ears. According to the correlation between expression intensity and yield, 33 proteins were identified. Proteins with
important contribution to yield, including starch synthases, seed storage proteins, and important components of these yields,
were verified by enzyme activity and yield formation for glycosidic synthetase and its gene regulatory network. The cloning
of important agronomic trait genes and the reconstruction of gene networks were discussed. |
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