| 费亚芬1,2
，王玉婷1,2
，陆夏逸1,2
，劳 梦1,2
，李颖豪1,2
，张拥军1,2*.海藻膳食纤维提取工艺优化及其对小鼠肠道菌益生
作用[J].海南师范大学学报自科版,2019,32(1):54-63 |
| 海藻膳食纤维提取工艺优化及其对小鼠肠道菌益生
作用 |
| Extraction and Optimization of Marine Algae Dietary Fiber and ItsEffects on Mice Intestinal Microflora |
| |
| DOI：10.12051/j.issn.1674-4942.2019.01.010 |
| 中文关键词: 海藻 龙须菜 膳食纤维 响应面优化 肠道菌群 |
| 英文关键词: marine algae dietary Gracilaria lemaneiformis response surface methodology intestinal microflora |
| 基金项目:“十二五”农村领域国家科技计划课题资助项目（2013BAD10B02-06） |
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| 摘要点击次数: 617 |
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| 中文摘要: |
| 为优化海藻膳食纤维的提取工艺，探究其对小鼠肠道菌的益生作用，采用《食品安全国
家标准 食品中膳食纤维的测定》（GB5009.88-2014），测得海带、裙带菜、海青菜、石花菜、龙须菜、坛
紫菜、条斑紫菜七种海藻的总膳食纤维含量。结合pH变化探究其对有益菌的促生作用，筛得龙须
菜为促进有益菌生长的最优海藻，其总膳食纤维含量为67.17%。采用单因素实验和响应面优化
法，得到超声波提取龙须菜非可溶性膳食纤维（Insoluble Dietary Fiber，IDF）中残存的可溶性膳食纤
维（Soluble Dietary Fiber，SDF）的最佳工艺条件：液料比（mL/g）95：1、时间 50 min、温度 50℃、功率
200 W，在此提取工艺条件下龙须菜IDF中残留SDF的得率为8.23%。以龙须菜总膳食纤维（Total
Dietary Fiber，TDF）、SDF为实验组，魔芋粉为阳性对照，探究其对抗生素造模肠道菌群失衡小鼠肠
道菌群的恢复效果，短期研究发现实验组能够改善造模鼠肠道菌群丰度，利于有益菌增长和肠道
环境恢复。研究结果填补了龙须菜膳食纤维（Dietary Fiber，DF）对肠道菌群益生作用研究的空白，
可为海藻膳食纤维综合利用提供参考。 |
| 英文摘要: |
| The purpose of this paper was to screen the optimal algal varieties using the yield of algal dietary fiber and the
effect of beneficial bacteria as the evaluation index, and explore the effect of the algal dietary fiber on the intestinal flora of
mice. The total dietary fiber (Total Dietary Fiber, TDF) content of seven kinds of marine algae, such as Laminaria japonica,
Undaria pinnatiflda, Ulva lactuca, Gelidium amansii, Gracilaria lemaneiformis, Porphyra haitanensis, Porphyra yezoensis,
was obtained using the national standard method (GB5009.88-2014). Combined with pH changes in mice enteric bacteria,
Gracilaria lemaneiformis was selected as the optimal algal, and its total dietary fiber content was 67.17%. The soluble dietary fiber (SDF) in Gracilaria lemaneiformis was extracted by ultrasonic method using the response surface optimization
based on the single factor experiment. The optimum ultrasonic conditions were shown as follows: the liqid-solid ratio of 95:
1 (mL/g), time 50 min, temperature 50 ℃, and power 200 W. Under the extraction conditions, the yield of residual SDF in
G. lemaneiformis IDF was 8.23%. The recovery effect of G. lemaneiformis TDF and SDF to the imbalanced intestinal micro⁃
flora mice induced by antibiotics was explored with Amorphophallus konjac powder used as the positive control. The results
indicated that the abundance of intestinal flora was improved in the mice model in short time, which facilitates the growth of
beneficial bacteria and restores the intestinal environment. The results of this experiment filled the blank of the study on
the probiotic effects of DF of G. lemaneiformis on mice intestinal microflora, providing a reference for the comprehensive uti⁃
lization of algal dietary fiber |
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