| 杨叶东1
，周丽春2
，徐志南2.不同饲料组分作为固定化细胞载体制备丁酸纳饲料
添加剂的可行性研究[J].海南师范大学学报自科版,2019,32(1):48-53 |
| 不同饲料组分作为固定化细胞载体制备丁酸纳饲料
添加剂的可行性研究 |
| Potential Evaluation of Feedstuff as Cell Immobilization Carrier forthe Preparation of Sodium Butyrate as Feed Additive |
| |
| DOI：10.12051/j.issn.1674-4942.2019.01.009 |
| 中文关键词: 丁酸 米糠 外置式纤维床反应器 吸附 |
| 英文关键词: butyric acid rice bran immobilization carrier adsorption |
| 基金项目:杭州市科技项目（20150435B22） |
|
| 摘要点击次数: 502 |
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| 中文摘要: |
| 本研究将米糠作为固定化载体，构建了一种新型的外置式纤维床反应器，应用于固定
化发酵产丁酸实验。以酪丁酸梭菌为发酵菌株，进行了游离细胞和固定化细胞发酵比较。结果表
明，固定化细胞分批发酵丁酸生产率为0.48 g·L-1
·h-1
，比游离分批发酵提高了55%。重复分批发
酵8个批次的丁酸浓度平均值为21.05 g/L，可以稳定多批次运行。米糠对丁酸钠的吸附模拟实验
表明，米糠对丁酸钠的吸附量为0.02～0.08 g/g。固定化发酵后的米糠会吸附产物丁酸钠，吸附量
为0.105 g/g，这有利于将反应后的米糠直接用来生产含丁酸钠的饲料添加剂。 |
| 英文摘要: |
| The present work was carried out to explore the possibility of rice bran or wheat bran as cell immobilization car⁃
rier. Firstly, with systematical experiments, the addition amount of two different carriers was determined, meanwhile the
lowest stirring rate was obtained to maintain the fluidization of the added carriers in the fermentor. Secondly, the effects of
adsorption time, pH, butyrate concentration and carrier amount on immobilized biomass and butyrate were examined with
each carrier. In the conclusion, rice bran is suitable as an immobilization carrier for new technology exploration for the pro⁃
duction of butyrate-containing feed using immobilized fermentation. |
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