| 朱丹1，邹水洋2*，程春苗1，邱华贤2.超声波在γ-聚谷氨酸发酵工艺中的应用[J].海南师范大学学报自科版,2018,31(4):428-433 |
| 超声波在γ-聚谷氨酸发酵工艺中的应用 |
| Application of Ultrasonic in the Fermentation of γ-Polyglutamic Acid |
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| DOI：10.12051/j.issn.1674-4942.2018.04.013 |
| 中文关键词: γ-聚谷氨酸 超声波 发酵 提取 |
| 英文关键词: poly-γ-glutamic acid ultrasonic irradiation fermentation extraction |
| 基金项目:东莞市国际科技合作项目（2013508140001） |
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| 中文摘要: |
| 考察了枯草杆菌液体发酵过程中施加超声波刺激对γ-聚谷氨酸合成的影响，并对超
声波辅助水提法提取固体基质中γ-聚谷氨酸的效果进行了研究。结果表明，超声波在适当条件下
刺激枯草杆菌，对其液体发酵合成γ-聚谷氨酸有明显促进作用。当枯草杆菌培养12 h 后，在37℃、
50 W 超声功率下连续辐照1 h，γ-聚谷氨酸产量提高了24.76%。超声波辅助提取γ-聚谷氨酸的
提取率比常规工艺提高30%以上。优化的超声提取工艺条件为：超声波功率40 W，温度为70 ℃下
浸提30 min。 |
| 英文摘要: |
| In this study, the influence of ultrasonic stimulation on the synthesis of γ-polyglutamic acid in submerged fer⁃
mentation by Bacillus subtilis was investigated and the effect of ultrasonic-assisted water extraction of γ-polyglutamic acid
in solid substrates was also studied. The results indicated that the synthesis of γ-polyglutamic acid from Bacillus subtilis
can be promoted by ultrasonic with appropriate stimulation in the submerged fermentation. The production of γ-polyglutam⁃
ic acid increased by 24.76% when Bacillus subtilis was cultivated for 12 h and then irradiated by the ultrasonic at 37 ℃
with 50W for 1h. The extraction yield of γ-polyglutamic acid in ultrasonic-assisted extraction was increased by 30% com⁃
pared with the conventional extraction method. The optimum extraction was conducted under the conditions of ultrasonic
power 40 W at 70 ℃ for 30 min. |
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