张敏,李志英.不同中药总黄酮含量测定方法的选择研究[J].海南师范大学学报自科版,2017,30(3):307-310 |
不同中药总黄酮含量测定方法的选择研究 |
Research on Determination Method of Flavonoids ofDifferent Traditional Chinese Medicine |
投稿时间:2016-12-15 |
DOI:10.12051/j.issn.1674-4942.2017.03.011 |
中文关键词: 总黄嗣 中草药 测定方法 黄嗣结构 |
英文关键词: total flavonoid spectrophotometry Chinese herbs structure of flavonoids |
基金项目: |
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中文摘要: |
波辅助提取十种中药中的总黄嗣,以芦丁为标准品,用五种不同的测定方法探
讨不同中草药中总黄嗣的含量,研究黄嗣结构和测定方法之间的关系.结果表明:直接测定法
更适合甘草、银杏叶和陈皮;NaN02
-AI(N03 ) 3 法更适合于山桂和黄武; 差示分光光度法更
适合于野菊花、山桂、葛根和连翘; AICl3 法更适合于陈皮; 荧光光度法除山桂外, 其它中草药
测定均佳.根据总黄嗣含量的测定方法的原理探讨不同中药中黄嗣类物质的结构. |
英文摘要: |
In order to investigate the best assay method of total flavonoids and the relationship between flavonoids structure
and assay method , total flavonoids of ten traditional Chinese medicines were extracted by microwave technology and determined
by different methods. TIe results showed that the direct methods were suitable for testing the total flavonoids of Glycyrrhizα,
ura1ensis , leaves of Ginkgo bilobα and pericarpium citri reticulatae , the method of NaN02 - AI( N0 3 ) 3 was suitable
for Crataegus pinnatifidα, and Astragα, lus membranaceus , the method of differential spectrophotometry was suitable for Chrysαnthemum
indicum , Crataegus pinnatifidα, , Puerariα lobatα , and Forsythia suspensα. TIe method of AICl3 was suitable for
pericarpium citri reticulatae , and the method of fluorospectrophotometrγwas suitable for all apart from the hawthorn. The
determination of total flavonoids in the Chinese medicines was affected by various factors , such as the types of flavonoids,
the choice of measurement method , etc. |
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