| 龚建如,王俊慧,蒙瑜,张娟,张文飞.新型Vip3 -like 蛋白的从头预测建模
及其结构域分析[J].海南师范大学学报自科版,2017,30(3):269-275 |
| 新型Vip3 -like 蛋白的从头预测建模
及其结构域分析 |
| Initio Prediction Modeling of Novel Vip3 -like and Its Domains Analysis |
| 投稿时间：2017-07-06 |
| DOI：10.12051/j.issn.1674-4942.2017.03.005 |
| 中文关键词: 营养期杀虫蛋白 苏云金芽抱杆菌 从头预测法 结构域 |
| 英文关键词: vegetative insecticidal proteins Bαcillus thuringiensis initio modeling domain |
| 基金项目: |
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| 中文摘要: |
| Vip3 -like 蛋白对小菜娥等鳞翅目昆虫有杀虫活性， 是苏云金芽抱杆菌营养期
杀虫蛋白Vip3的同源蛋白.为建立Vip3 -like 三维结构的理论模型并对其结构域进行生物信
息学分析，选择从头预测的方法对Vip3 -like 蛋白三维结构的理论模型进行预测分析. 分析结
果表明，Vip3 -like 蛋白由1 个Vip3A -N 超家族结构域和3 个糖类结合结构域组成. 与模式
蛋白Vip3 Aa相比，Vip3 -like 是一种分子量更大的蛋白质， 且2 种蛋白的表面静电势分布也
存在差异.其Vip3 A - N 结构域仅由α 螺旋和无规则卷曲组成， 没有发现自折叠. 3 个糖类结
合结构域主要由无规则卷曲和自折叠组成，仅存在1个α螺旋结构.结构域分析预示无规则
卷曲和自折叠可能在Vip3 -like 和受体结合的过程中扮演着重要角色. 通过比较分析， 发现
Vip3Aa 和Vip3 -like 蛋白在三级结构上存在较大差异， 仅仅在局部结构存在一定的相似性，
Vip3 -like 可能并不属于Vip3Aa 类蛋白家族而属于一种全新的Bt 营养期杀虫蛋白质. |
| 英文摘要: |
| The Vip3 -like protein with the insecticidal activity against Lepidoptera larvae of Plutellαxylostellα i s a homologous
vegetative insecticidal proteins produced by some Bacillus thuringiensis strains. To investigate the theoretical model of
the three - dimensional structure of diamondback mothcidal Vip3 -like and its bioinformatics analysis , the theoretical model
of Vip3Aa and Vip3 -like was predicted by ab initio prediction modeling by the Phyre2 engine with the intensive mode.
咀Ie results show that Vip3 - like protein is a bigger molecule than vip3Aa composed of four distinct domains , and the
Vip3A - N domain only consists ofα- helix and loop structure without any 13 - sheet. As for the carbohydrate binding domains
, only one α- helix is found in three carbohydrate binding domains , and the distribution of electrostatic potential of
the two toxins is different. The 3D structure of Vip3 -like does not resemble the Vip3Aa structures very well but still shows
some similarity which indicates the Vip3 -like may belong to a new Vip super faIllily. Domain analysis indicates that the 13
- sheet and loop structure may play an important role during the binding process. |
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