| 冯梦, 冯渴芯, 魏力.珊瑚共生虫黄藻对高浓度CO2的响应机制[J].海南师范大学学报自科版,2026,(2):243-253 |
| 珊瑚共生虫黄藻对高浓度CO2的响应机制 |
| Response Mechanism of Coral Symbiotic Symbiodinium microadriaticum to High CO2 Concentration |
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| DOI：10.12051/j.issn.1674-4942.2024.04.015 |
| 中文关键词: 珊瑚共生虫黄藻 二氧化碳 转录组 代谢通路 海洋酸化 碳浓缩机制 |
| 英文关键词: coral symbiotic carbon dioxide transcriptome metabolic pathways ocean acidification carbon-concentrating mechanism |
| 基金项目:国家自然科学基金项目（32360022， 32160020） |
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| 摘要点击次数: 175 |
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| 中文摘要: |
| 人类活动导致大气 CO2 浓度持续攀升，海洋吸收大量CO2引发海水酸化，严重威胁珊瑚-虫黄藻共生体的健康，常导致虫黄藻流失与珊瑚白化。为探究珊瑚共生虫黄藻对海水酸化的分子响应机制，本研究重点关注碳浓缩机制（carbon-concentrating mechanism， CCM）、能量代谢及氧化应激等关键通路，以虫黄藻（Symbiodinium microadriaticum）为研究对象，设置高浓度CO2（5%）与常规空气（0.04%）2组处理，分别于24、48、72 h取样进行转录组测序。结果显示，高浓度CO2处理下虫黄藻差异表达基因数量呈先增后减特征，24 h鉴定出82个差异基因，48 h达峰值144个，72 h锐减至11个，表明虫黄藻在胁迫中期转录响应最剧烈，其后逐步建立新稳态；富集分析揭示，上述差异基因主要参与能量代谢（三羧酸循环、氧化磷酸化）、氧化应激响应（谷胱甘肽代谢）以及跨膜运输等关键生物学通路。本研究表明高浓度CO2引发虫黄藻复杂的转录重编程，为理解珊瑚共生体的环境适应性提供了分子依据。 |
| 英文摘要: |
| Human activities have led to a continuous rise in atmospheric CO2 concentrations, while the ocean absorbs large amounts of CO2, triggering seawater acidification that severely threatens the health of the coral-symbiotic Symbiodinium microadriaticum ecosystem, often resulting in zooxanthellae loss and coral bleaching. To investigate the molecular response mechanisms of coral symbiotic dinoflagellates to seawater acidification, this study focused on key pathways such as the carbon-concentrating mechanism (CCM), energy metabolism, and oxidative stress, using S. microadriaticum as the research subject. Two treatment groups were established: high CO2 concentration (5%) and conventional air (0.04%), with transcriptome sequencing samples collected at 24, 48, and 72 hours. The results showed that the number of differentially expressed genes in S. microadriaticum under high CO2 treatment exhibited an initial increase followed by a decline. 82 differentially expressed genes were identified at 24 hours, peaked at 144 genes at 48 hours, and sharply decreased to 11 genes at 72 hours, indicating that transcriptional response during the mid-stress phase was the most intense, followed by gradual establishment of a new homeostasis. Enrichment analysis revealed that these differentially expressed genes primarily participate in critical biological pathways such as energy metabolism (tricarboxylic acid cycle, oxidative phosphorylation), oxidative stress response (glutathione metabolism) and transmembrane transport. This study demonstrates that high CO2 concentrations induce complex transcriptional reprogramming in S. microadriaticum, providing a molecular basis for understanding the environmental adaptability of coral symbionts. |
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