| 陈帝君, 杜艳, 熊慧, 杨光忠, 陈玉.打箭菊萜类成分研究[J].海南师范大学学报自科版,2026,(2):163-168 |
| 打箭菊萜类成分研究 |
| Study on Terpenoid Constituents from Tanacetum tatsienense |
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| DOI：10.12051/j.issn.1674-4942.2024.04.006 |
| 中文关键词: 打箭菊 萜类成分 去胡萝卜素苷 抗增殖活性 |
| 英文关键词: terpenoid norcarotenoid glycoside anti-proliferative activity |
| 基金项目:国家重点研发计划项目（2022YFC3502200） |
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| 摘要点击次数: 772 |
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| 中文摘要: |
| 本文采用系统溶剂提取法、各种色谱技术（正反相硅胶柱层析以及半制备高效液相色谱等）对打箭菊（Tanacetum tatsienense）乙酸乙酯提取物的化学成分进行分离纯化。通过各种波谱技术（1D和2D-NMR）、高分辨质谱、量子化学的计算以及化学方法对所分离的化合物进行结构鉴定，分离并鉴定6个萜类化合物，其中化合物1为新去胡萝卜素苷，命名为tatsienenoside A （1），其余的已知化合物分别鉴定为3β-hydroxy-β-ionone （2）、1β-hydroxy-7-epi-costic acid methyl ester （3）、阿里二醇（4）、款冬二醇 （5）、马尼拉二醇 （6）。采用CCK-8方法对所分离的萜类化合物进行体外抗肿瘤细胞增殖活性测试，结果表明阿里二醇对人非小细胞肺癌A549细胞有较好的抗增殖活性，IC50值为（12.58±0.60） µmol/L。 |
| 英文摘要: |
| In this study, systematic solvent extraction techniques and different chromatographic methods, such as normal-phase and reverse-phase silica gel column chromatography, along with semi-preparative high-performance liquid chromatography, were employed to isolate and purify the chemical components extracted from the ethyl acetate extract of Tanacetum tatsienense. The structures of the obtained compounds were determined using a range of spectroscopic techniques, including 1D and 2D NMR, high-resolution mass spectrometry, quantum chemical calculations, and conventional chemical methods. Six terpenoids were isolated and identified. Among them, compound 1 was recognized as a new norcarotenoid glycoside, named as tatsienenoside A （1）. The remaining compounds were identified as known terpenoids, including 3β-hydroxy-β-ionone （2）, 1β-hydroxy-7-epi-costic acid methyl ester （3）, arnidiol （4）, faradiol （5）, and maniladiol （6）. The anti-proliferative activity of the isolated terpenoids was assessed using the CCK-8 method. The results indicated that arnidiol （4） exhibited moderate anti-proliferative activity against human non-small cell lung cancer A549 cells, with an IC50 value of （12.58 ± 0.60） µmol/L. |
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