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| 不同部位金花葵黄酮提取工艺及抗氧化性的比较研究 |
| Comparative study on extraction technology and antioxidant activity of Flavonoids from Different Parts of Abelmoschus manihot (L.)Medicus |
| 投稿时间：2026-01-19 修订日期：2026-03-07 |
| DOI： |
| 中文关键词: 金花葵 黄酮 响应面优化 微波提取法 抗氧化 |
| 英文关键词: Abelmoschus manihot (L.) Medicus, flavonoids, response surface optimization, microwave extraction, antioxidant activity |
| 基金项目:2024年度河南省高等学校重点科研项目(24B550010)；2025年度河南省高等学校重点科研项目(24B550027)；2025年度河南省科技攻关项目(252102110084) |
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| 中文摘要: |
| 本研究以不同部位金花葵为主要原料,采用单因素结合响应面法对水浴预处理辅助微波提取不同部位金花葵中的黄酮工艺进行优化,分析不同部位所得黄酮的抗氧化活性。结果表明,花部在微波时间60s,液料比57(mL:g),乙醇浓度56%条件下,黄酮得率为2.7956%；叶部在微波时间51s,液料比28(mL:g),乙醇浓度45%条件下,黄酮得率为1.9795%；茎部在微波时间61s,液料比48(mL:g),乙醇浓度52%条件下,黄酮得率为0.3952%；根部在微波时间64s,液料比38(mL:g),乙醇浓度60%条件下,黄酮得率为0.2338%。体外抗氧化试验结果表明,DPPH试验中,当花、叶、茎、根的浓度为21μg/ml时,其清除率分别为95.1%、61.1%、69.7%、93.9%；FRAP试验中,当花、叶、茎、根的浓度为80μg/ml时,其FRAP值分别为0.9652,0.5259,0.3943,0.3863。ABTS试验中,当花、叶、茎、根浓度为12μg/ml时,其清除率分别为89.5%、71.2%、80.1%、82.3%；总还原力试验中,当花、叶、茎、根浓度为70μg/ml,其还原力值分别为0.863,0.257,0.281,0.654。综合对比可知,金花葵不同部位的黄酮得率与抗氧化活性均存在明显层级差异,其中花的黄酮得率及抗氧化性表现最佳,根与茎的抗氧化性表现接近,叶的抗氧化性相对最差。本研究结果表明优化后的水浴预处理辅助微波提取不同部位金花葵工艺参数可靠,提取得到的黄酮具有较为理想的抗氧化活性,为金花葵黄酮定向开发与高值化利用提供了数据支撑。 |
| 英文摘要: |
| This study was aimed to optimize the water bath pretreatment-assisted microwave extraction process of flavonoids from different parts of Abelmoschus manihot (L.) Medicus using single-factor experiments combined with response surface methodology, and to analyze the antioxidant activities of flavonoids obtained from different parts. The results showed that the flavonoid yield from flowers reached 2.7956% under the conditions of microwave time 60s, liquid-to-material ratio 57:1 (mL:g), and ethanol concentration 56%; the yield from leaves was 1.9795% under microwave time 51s, liquid-to-material ratio 28:1 (mL:g), and ethanol concentration 45%; the yield from stems was 0.3952% under microwave time 61s, liquid-to-material ratio 48:1 (mL:g), and ethanol concentration 52%; and the yield from roots was 0.2338% under microwave time 64s, liquid-to-material ratio 38:1 (mL:g), and ethanol concentration 60%. In vitro antioxidant assays demonstrated that, in the DPPH radical scavenging assay, the scavenging rates of flavonoids from flowers, leaves, stems, and roots were 95.1%, 61.1%, 69.7%, and 93.9% at a concentration of 21 μg/mL, respectively; in the FRAP assay, the corresponding FRAP values were 0.9652, 0.5259, 0.3943, and 0.3863 at a concentration of 80 μg/mL; in the ABTS radical scavenging assay, the scavenging rates were 89.5%, 71.2%, 80.1%, and 82.3% at a concentration of 12 μg/mL; and in the total reducing power assay, the reducing power values were 0.863, 0.257, 0.281, and 0.654 at a concentration of 70 μg/mL. A comprehensive comparison revealed significant hierarchical differences in both flavonoid yield and antioxidant activity among different parts of Abelmoschus manihot (L.) Medicus. The flowers exhibited the highest flavonoid yield and the strongest antioxidant activity, the roots and stems showed comparable antioxidant performance, and the leaves had the relatively weakest antioxidant activity. The results of this study indicate that the optimized parameters of water bath pretreatment-assisted microwave extraction for different parts of Abelmoschus manihot (L.) Medicus are reliable, and the extracted flavonoids possess desirable antioxidant activity, providing data support for the targeted development and high-value utilization of Abelmoschus manihot (L.) Medicus flavonoids. |
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