| 覃天涵, 覃钰岚, 邓培颖, 黄思敏, 朱英芝.沙雷氏菌属菌株GXMZU-S产天然色素特性及应用[J].海南师范大学学报自科版,2026,(1):81-87 |
| 沙雷氏菌属菌株GXMZU-S产天然色素特性及应用 |
| Characterization and Application of Natural Pigments Produced by Serratia sp.Strain GXMZU-S |
| |
| DOI：10.12051/j.issn.1674-4942.2024.04.011 |
| 中文关键词: 为获得具有产色素潜力的微生物资源，本研究以广西玉林大容山森林自然保护区土壤为样本开展了产色素微生物的分离与筛选工作，成功获得一株具有显著紫色天然色素合成能力的细菌GXMZU-S。通过16S rDNA基因序列系统发育分析，初步鉴定该菌株为粘质沙雷氏菌（Serratia marcescens）。研究发现，采用静置培养方式、以麦芽糖为碳源、以硝酸钾为氮源可有效提升天然色素产量。该微生物源天然色素在酸性和中性条件下均能保持良好色泽，具有优异的热稳定性，在20 ~ 70 ℃范围内能维持较高的色价。将其用于棉纺织物染色时，所得染色样品的耐水色牢度与耐摩擦色牢度均可达4 ~ 5级，显示出优良的实用性能。研究结果验证了GXMZU-S色素在纺织染色领域的应用潜力，丰富了产色素微生物资源库，为微生物源天然色素的应用提供了一定的实践参考。 |
| 英文关键词: 粘质沙雷氏菌 微生物源天然色素 培养条件优化 色素稳定性 |
| 基金项目:广西大学生创新训练计划项目（S202410608010） |
|
| 摘要点击次数: 467 |
| 全文下载次数: 108 |
| 中文摘要: |
| 为获得具有产色素潜力的微生物资源，本研究以广西玉林大容山森林自然保护区土壤为样本开展了产色素微生物的分离与筛选工作，成功获得一株具有显著紫色天然色素合成能力的细菌GXMZU-S。通过16S rDNA基因序列系统发育分析，初步鉴定该菌株为粘质沙雷氏菌（Serratia marcescens）。研究发现，采用静置培养方式、以麦芽糖为碳源、以硝酸钾为氮源可有效提升天然色素产量。该微生物源天然色素在酸性和中性条件下均能保持良好色泽，具有优异的热稳定性，在20 ~ 70 ℃范围内能维持较高的色价。将其用于棉纺织物染色时，所得染色样品的耐水色牢度与耐摩擦色牢度均可达4 ~ 5级，显示出优良的实用性能。研究结果验证了GXMZU-S色素在纺织染色领域的应用潜力，丰富了产色素微生物资源库，为微生物源天然色素的应用提供了一定的实践参考。 |
| 英文摘要: |
| To obtain microbial resources with the potential for producing microbially derived natural pigments, this study conducted the isolation and screening of pigment-producing microorganisms using soil samples from the Dalongshan Forest Nature Reserve in Yulin, Guangxi. A bacterial strain, designated as GXMZU-S, demonstrating a remarkable ability to synthesize natural purple pigments, was successfully isolated. Phylogenetic analysis based on 16S rDNA gene sequences preliminarily identified the strain as Serratia marcescens. The culture conditions for pigment production by this strain were further optimized. Strategies including the adoption of a static cultivation method, and the identification of maltose as the optimal carbon source combined with potassium nitrate as the optimal nitrogen source, were implemented, effectively enhancing the yield of the microbially derived natural pigment. A systematic assessment of its physicochemical stability was subsequently performed. The pigment maintained favorable coloration under both acidic and neutral conditions, exhibited excellent thermal stability, and retained a high color value within the temperature range of 20 ℃ to 70 ℃. When applied for dyeing cotton textiles, the dyed fabrics achieved both washing fastness and rubbing fastness ratings of 4-5, demonstrating superior practical performance. The results validate the application potential of the GXMZU-S pigment in the field of textile dyeing, enrich the library of microbial resources capable of producing microbially derived natural pigments, and provide practical references for the application of such pigments. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
