| 张思晨, 汪捷, 胡磊, 王慧.三种近红外荧光探针的合成及其对粘度的检测[J].海南师范大学学报自科版,2026,(1):27-33 |
| 三种近红外荧光探针的合成及其对粘度的检测 |
| Synthesis of Three Near-infrared Fluorescent Probes and Their Detection of Viscosity |
| |
| DOI：10.12051/j.issn.1674-4942.2024.04.004 |
| 中文关键词: 线粒体是细胞内重要的能量提供者，参与细胞代谢和信号传递等关键生物过程。线粒体功能异常可导致细胞功能紊乱，严重时可能引发多种疾病。线粒体粘度变化是评估线粒体功能的重要指标，具有重要的研究意义。基于这一需求，本研究以苯并噻唑衍生物为核心结构，引入吡啶盐和喹啉盐，设计合成了3种具有D-π-A构型的荧光探针BTZ1 ~ BTZ3，并进一步研究了探针的光物理性质及生物学性质。实验结果表明，探针BTZ1和BTZ3对溶液粘度变化具有较好的响应，探针BTZ1 ~ BTZ3均表现出良好的光稳定性和较低的细胞毒性，探针BTZ3可有效监测线粒体内粘度变化。 |
| 英文关键词: 苯并噻唑 粘度 细胞成像 荧光探针 |
| 基金项目:安徽省高校自然科学研究项目（2023AH051762，2023AH051747）；安徽省大学生创新创业教育训练计划项目（S202410368090，S202410368088） |
|
| 摘要点击次数: 653 |
| 全文下载次数: 112 |
| 中文摘要: |
| 线粒体是细胞内重要的能量提供者，参与细胞代谢和信号传递等关键生物过程。线粒体功能异常可导致细胞功能紊乱，严重时可能引发多种疾病。线粒体粘度变化是评估线粒体功能的重要指标，具有重要的研究意义。基于这一需求，本研究以苯并噻唑衍生物为核心结构，引入吡啶盐和喹啉盐，设计合成了3种具有D-π-A构型的荧光探针BTZ1 ~ BTZ3，并进一步研究了探针的光物理性质及生物学性质。实验结果表明，探针BTZ1和BTZ3对溶液粘度变化具有较好的响应，探针BTZ1 ~ BTZ3均表现出良好的光稳定性和较低的细胞毒性，探针BTZ3可有效监测线粒体内粘度变化。 |
| 英文摘要: |
| Mitochondria are important energy providers in cells and are involved in key biological processes such as cellular metabolism and signaling. Abnormal mitochondrial function can lead to cellular dysfunction, which may cause a variety of diseases in severe cases. The change of mitochondrial viscosity is an important indicator for assessing mitochondrial function, which is of great research significance. Based on this need, three fluorescent probes BTZ1-BTZ3 with D-π-A configuration were designed and synthesized by using benzothiazole derivatives as the core structure and introducing pyridinium and quinoline salts, and the photophysical and biological properties of the probes were further investigated. Experimental results indicated that probes BTZ1 and BTZ3 respond well to changes in the viscosity of the solution, and all of them exhibit good photostability and low cytotoxicity. In addition, probe BTZ3 can effectively monitor viscosity changes in mitochondria. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
