文章摘要
杏仁磨浆渣中残留蛋白溶出方法及工艺优化研究
Study on Dissolution Methods and Process Optimization of Residual Protein in Apricot Kernel Grinding Residue
投稿时间:2025-11-26  修订日期:2026-02-05
DOI:
中文关键词: 杏仁磨浆渣  蛋白溶出率  蛋白质结构  工艺优化
英文关键词: Apricot kernel residue  protein  extraction yield  structure  parameter optimization
基金项目:
作者单位邮编
胡恩浩 陕西师范大学食品工程与营养科学学院 710062
袁文慧 陕西师范大学食品工程与营养科学学院 
田柏慧 陕西师范大学食品工程与营养科学学院 
兰 怡 陕西师范大学食品工程与营养科学学院 
张清安* 陕西师范大学食品工程与营养科学学院 710062
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中文摘要:
      本文以杏仁磨浆剩余渣为原料,比较了5种不同处理方法(水提法、碱液法、纤维素酶酶解法、超声法和超声辅助纤维素酶酶解法)对杏仁磨浆渣中残余蛋白溶出率以及溶出蛋白结构与表面疏水性的影响;在筛选出较好方法和单因素实验的基础上,采用响应面优化试验探究超声辅助纤维素酶酶解法促进杏仁磨浆渣中蛋白质溶出的最佳工艺参数。结果表明,在处理时间为30 min时,5种处理方法对应磨浆渣时中蛋白质的溶出率分别为4.79%、14.76%、15.41%、25.38%和35.38%,超声辅助纤维素酶酶解法处理效果显著优于其他方法。响应面优化超声辅助纤维素酶促进杏仁磨浆渣中蛋白质溶出的最佳工艺为:纤维素酶添加量650 U/g、料液比1:23 (g/mL)、超声功率240 W、超声处理时长41 min,此条件下实际提取得率为41.94%。研究结果可为工厂化杏仁磨浆工艺改进、蛋白高效利用提供重要指导。
英文摘要:
      This study used the residual residue from apricot kernel grinding as the raw material to compare the effects of five different treatment methods—water extraction, alkaline extraction, cellulase hydrolysis, ultrasonic treatment, and ultrasonic-assisted cellulase hydrolysis—on the dissolution rate of residual protein in the apricot kernel grinding residue, as well as on the structure and surface hydrophobicity of the dissolved protein. On the basis of screening out the optimal method and conducting single-factor experiments, response surface optimization experiments were adopted to explore the optimal process parameters for promoting protein dissolution from the apricot kernel grinding residue using the ultrasonic-assisted cellulase method. The results showed that when the treatment time was 30 min, the protein dissolution rates of the five methods were 4.79%, 14.76%, 15.41%, 25.38%, and 35.38%, respectively; the ultrasonic-assisted cellulase method exhibited a significantly better effect than the other methods. The optimal process for promoting protein dissolution from the apricot kernel grinding residue via the ultrasonic-assisted cellulase method, optimized by response surface methodology, was as follows: cellulase addition amount of 650 U/g, solid-liquid ratio of 1:23 (g/mL), ultrasonic power of 240 W, and ultrasonic treatment duration of 41 min. Under these conditions, the actual extraction yield reached 41.94%. The research results can provide important guidance for the improvement of industrial apricot kernel grinding processes and the efficient utilization of proteins.
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