| 秦 潇，韩明钊，刘 新，李贵响，李 鹏*.铁载体在青枯菌与根际微生物群落互作中的
关键作用[J].海南师范大学学报自科版,2025,38(4):444-453 |
| 铁载体在青枯菌与根际微生物群落互作中的
关键作用 |
| Key Role of Siderophore in the Interaction Between Ralstonia solanacearum and the Rhizosphere Microbial Community |
| |
| DOI：10.12051/j.issn.1674-4942.2025.04.010 |
| 中文关键词: 茄雷尔氏菌 铁载体 根际微生物群落 16S RNA测序 微生物多样性 |
| 英文关键词: Ralstonia solanacearum siderophores rhizosphere microbial community 16S rRNA gene sequencing microbial diversity |
| 基金项目:国家自然科学基金项目（32260652） |
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| 摘要点击次数: 331 |
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| 中文摘要: |
| 青枯菌（Ralstonia solanacearum）可引起茄科作物发生毁灭性青枯病，铁载体对其获取
铁资源及入侵宿主至关重要。在番茄根际接种野生型青枯菌EP1及其铁载体合成缺失突变体，
利用16S rRNA测序技术分析番茄根际土壤细菌群落结构。结果表明，接种野生型改变了根际土
壤细菌群落结构；接种突变体后，茄雷尔氏菌属（Ralstonia）定殖丰度显著降低（P < 0.05），细菌群
落 α 多样性显著提高（P < 0.05），其群落组成结构更接近于健康土壤；共现网络分析结果显示野
生型青枯菌促进特定假单胞菌的富集，而突变体处理下酸杆菌门（Acidobacteriota）等寡营养菌丰
度提升。综上可推测，铁载体缺失削弱了青枯菌的铁竞争优势，缓解了根际铁资源竞争压力，从
而重构微生物群落结构并增强系统稳定性。该研究为解析青枯菌-根际微生物互作机制提供了
新视角。 |
| 英文摘要: |
| Ralstonia solanacearum induces destructive bacterial wilt. Siderophores are crucial for its iron acquisition and
invasive capacity to host plant. This study investigated the impact of siderophores on rhizosphere microbial communities by
inoculating tomato roots with wild-type (WT) strain EP1 or its siderophore synthesis-deficient mutant. Rhizosphere soil
bacterial communities were analyzed using 16S rRNA gene sequencing. The results demonstrated that inoculation with the
wild-type strain (EP1) altered the rhizobacterial community structure. In contrast, inoculation with the mutant resulted in a
significant reduction (P < 0.05) in the colonization abundance of Ralstonia and a significant increase (P < 0.05) in
community alpha diversity, with the overall community structure closely resembling that of healthy counterparts. Further
co-occurrence network analysis revealed that the wild-type promoted the co-enrichment of specific Pseudomonas species,
whereas mutant treatment enhanced the abundance of oligotrophic taxa such as Acidobacteria. In summary, it was
concluded that siderophore deficiency could attenuate the iron competition advantage of R. solanacearum, and then
alleviate competitive pressure for iron resources in the rhizosphere, thereby restructuring the microbial community and
enhancing system stability. This study provides a new perspective for elucidating the interaction mechanisms between R.solanacearum and rhizosphere microorganisms. |
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