| Various separation techniques including normal and adverse silica gel and Sephadex LH-20 gel column chram‐
atography were used to investigate the chemical constituents from Euphorbia fischeriana Steud. Finally, sixteen compounds,
including (-)-3,3′-bisdemethylpinoresinol (1), 7-epi-sesamin- dicatechol (2), syringaresinol (3), (+)-lariciresinol-9-eico‐
sanoic acid (4), isoamericanin A (5), N-benzoyl-L-phenylalaninyl-N-benzoyl-L-phenylalaninate (6), (22E)-ergosta-6,9,
22-triene-3β,5β,8α-tiol (7), (22E,24R)-5α,8α-epidioxyergosta-6,22-diene-3β-ol (8), (22E,24R)-5α,8α-epidioxy-23-
methylergosta- 6,22- diene-3β-ol (9), β-sitosterol(10), Schleicheol 1 (11), 3′-(4′′-hydroxy-3′′-methoxyphenyl)-propyl
benzoate (12), 3-hydroxybenzene ethanol (13), N-benzoyl-L-phenyla-laninol (14), crypticin B (15), gallic acid (16) were
isolated, and their structures were identified by NMR data and comparison with values reported. Compounds 1, 2, 4‒7, 9,
and 12 were isolated from the genus Euphorbia for the first time. Except for 10 and 16, the others were firstly isolated from
E. fischeriana Steud. Compound 4 showed obvious inhibitory activity against breast cancer cell line (MCF-7) with IC50 of
(11.3 ± 0.34) μmol/L [cisplatin IC50 = (6.2 ± 0.20) μmol/L]. |
