| 何晓梅, 赵文慧, 杨康龙, 余荧, 高影.黄精提取物对α-葡萄糖苷酶的抑制作用[J].海南师范大学学报自科版,2026,36(1):65-71 |
| 黄精提取物对α-葡萄糖苷酶的抑制作用 |
| α-Glucosidase Inhibitory Activity of the Extracts from Polygonatum sibiricum |
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| DOI：10.12051/j.issn.1674-4942.2024.04.009 |
| 中文关键词: 为挖掘黄精中抑制α-葡萄糖苷酶的活性物质并明确其作用机制，本研究采用水提和80%乙醇提取2种方法，对相同质量的黄精粉末进行提取，经减压浓缩、冷冻干燥后获得水提取物与醇提取物干品 通过测定2种提取物中多糖、多酚和黄酮类生物活性物质的含量，结合α-葡萄糖苷酶抑制活性检测分析活性物质含量与酶活抑制率的相关性，并对抑制作用较强的提取物进行酶促动力学分析以明确其抑制类型。结果显示：黄精水提取物得率（63.56%）高于醇提取物（56.72%） 水提取物中多糖含量（74.92%）显著高于醇提取物（48.80%），而醇提取物中的多酚（0.510%）和黄酮（0.021%）含量均高于水提取物（分别为0.380%、0.003%） 醇提取物对α-葡萄糖苷酶的抑制作用强于水提取物，但弱于对照品阿卡波糖，酶促动力学分析表明其抑制类型为反竞争性抑制。本研究为黄精中α-葡萄糖苷酶抑制活性物质的筛选与开发提供了理论依据和实验支撑。 |
| 英文关键词: 黄精提取物 生物活性物质 -葡萄糖苷酶 抑制作用 酶促动力学 |
| 基金项目:安徽省高校自然科学研究重点项目（2024AH051992）；国家级大学生创新训练项目（202410376009）；安徽省大学生创新训练项目（S202310376057） |
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| 中文摘要: |
| 为挖掘黄精中抑制α-葡萄糖苷酶的活性物质并明确其作用机制，本研究采用水提和80%乙醇提取2种方法，对相同质量的黄精粉末进行提取，经减压浓缩、冷冻干燥后获得水提取物与醇提取物干品；通过测定2种提取物中多糖、多酚和黄酮类生物活性物质的含量，结合α-葡萄糖苷酶抑制活性检测分析活性物质含量与酶活抑制率的相关性，并对抑制作用较强的提取物进行酶促动力学分析以明确其抑制类型。结果显示：黄精水提取物得率（63.56%）高于醇提取物（56.72%）；水提取物中多糖含量（74.92%）显著高于醇提取物（48.80%），而醇提取物中的多酚（0.510%）和黄酮（0.021%）含量均高于水提取物（分别为0.380%、0.003%）；醇提取物对α-葡萄糖苷酶的抑制作用强于水提取物，但弱于对照品阿卡波糖，酶促动力学分析表明其抑制类型为反竞争性抑制。本研究为黄精中α-葡萄糖苷酶抑制活性物质的筛选与开发提供了理论依据和实验支撑。 |
| 英文摘要: |
| To explore the α-glucosidase inhibitory active substances in Polygonatum sibiricum and clarify their mechanism of action, two extraction methods, water extraction and 80% ethanol extraction, were adopted to extract the same mass of Polygonatum sibiricum powder. After vacuum concentration and freeze-drying, dry products of water extract and ethanol extract were obtained. The contents of main bioactive substances including polysaccharides, polyphenols and flavonoids in the two extracts were determined. Combined with the determination of α-glucosidase inhibitory activity, the correlation between active substance contents and enzyme inhibition rate was analyzed. The extract with stronger inhibitory effect was subjected to enzymatic kinetic analysis to clarify its inhibitory type. The results showed that the yield of water extract (63.56%) was higher than that of ethanol extract (56.72%). The polysaccharide content in water extract (74.92%) was significantly higher than that in ethanol extract (48.80%), while the contents of polyphenols (0.510%) and flavonoids (0.021%) in ethanol extract were higher than those in water extract (0.380% and 0.003%, respectively). The ethanol extract exhibited stronger α-glucosidase inhibitory activity than the water extract, but weaker than the positive control acarbose. Enzyme kinetic analysis indicated that its inhibition type was uncompetitive inhibition. This study provides theoretical basis and experimental support for the screening and development of α-glucosidase inhibitory active substances in Polygonatum sibiricum. |
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